BPoD has moved!

BPoD has recently changed our domain name - we can now be found at bpod.org.uk

Please update your bookmarks!

Now in our 12th year of bringing you beautiful imagery from biomedical science every day

Search the archive of over 4000 images

Mutation Models

CRISPR gene editing to create liver organoids with mutations underlying fibrolamellar carcinoma

15 May 2023

Mutation Models

Fibrolamellar carcinoma (FLC) is an often fatal liver cancer affecting young people. The molecular mechanisms driving it are unclear, partly because of a lack of experimental models. Researchers now create human liver organoids (pictured using fluorescent microscopy) of healthy liver tissue (top, left) and, using genetic engineering technology, CRISPR, those that replicate liver with the different genetic faults found in FLC. Most common is the fusion of genes DNAJB1 and PRKACA (top, right), and also found are faults in PRKAR2A and BAP1 genes separately and together (bottom, left to right). FLC organoids mimicked tumour samples from patients and their liver cells regressed to an immature state. However, only faulty BAP1 and PRKAR2A together caused liver cells to turn into progenitor-like cells that, unlike normal liver cells, could grow in liver ducts. Meanwhile, DNAJB1-PRKACA organoids showed milder cancer features compared with other FLC organoids. Together, these FLC models hold promise for studying FLC progression.

Written by Lux Fatimathas

Search The Archive

Submit An Image

Follow on Tumblr

Follow on Instagram

What is BPoD?

BPoD stands for Biomedical Picture of the Day. Managed by the MRC Laboratory of Medical Sciences until Jul 2023, it is now run independently by a dedicated team of scientists and writers. The website aims to engage everyone, young and old, in the wonders of biology, and its influence on medicine. The ever-growing archive of more than 4000 research images documents over a decade of progress. Explore the collection and see what you discover. Images are kindly provided for inclusion on this website through the generosity of scientists across the globe.

Read More

BPoD is also available in Catalan at www.bpod.cat with translations by the University of Valencia.